Table of Contents

Noninvasive imaging for supporting basic research.
Noninvasive imaging in the pharmaceutical industry.
How to set up a small animal imaging unit.
Noninvasive small rodent imaging: significance for the 3R principles.
Institutional preconditions for small animal imaging.
Statistical considerations for animal imaging studies.
Animal anesthesia and monitoring.
Drug administration.
How to choose the right imaging modality.
X-ray and X-ray-CT.
CT contrast agents.
Small animal magnetic resonance imaging: basic principles, instrumentation and practical issue.
MR contrast agents.
MR spectroscopy.
Ultrasound.
Ultrasound contrast agents.
PET and SPECT. Radiotracer I: standard tracer.
Radiotracer II: peptide-based radiopharmaceuticals.
Optical imaging.
Optical probes.
Multi-modal imaging and image fusion.
Ex vivo and in vitro cross calibration methods.
In vitro methods for in vivo quantitation of PET and SPECT imaging probes: autoradiography and gamma counting.
Qualitative and quantitative data analysis.
Guidelines for nuclear image analysis.
Kinetic modeling.
Data documentation systems.
Imaging in developmental biology.
Imaging in gynecology research.
Imaging in cardiovascular research.
Imaging in neurology research I: neurooncology.
Imaging in neurology research II: PET imaging in CNS disorders.
Imaging in neurology research III: focus on neurotransmitter imaging.
Imaging in oncology research.
Imaging in immunology research. Small animal imaging has been recognized as an important tool in preclinical research. Nevertheless, the results of non-invasive imaging are often disappointing owing to choice of a suboptimal imaging modality and/or shortcomings in study design, experimental setup, and data evaluation. This textbook is a practical guide to the use of non-invasive imaging in preclinical research. Each of the available imaging modalities is discussed in detail, with the assistance of numerous informative illustrations. In addition, many useful hints are provided on the installation of a small animal unit, study.