Table of Contents

Frontmatter.
Section I: Introduction. Introduction to Pluripotent Stem Cells: Biology and Applications / Maria Borowski, Gary S Stein.
Researching and Obtaining Established Stem Cell Lines / Mai X Luong, Kelly P Smith, Gary S Stein.
Section II: Laboratory Guide for Human Stem Cell Culture: Pluripotent Stem Cell Culture. Basics of Cell Culture / Alicia Allaire, Mai X Luong, Kelly P Smith.
The Stem Cell Laboratory / Alicia Allaire, Mai X Luong, Kelly P Smith.
Reagent Preparation / Alicia Allaire, Mai X Luong, Kelly P Smith.
Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells / Meng-Jiao Shi, Maria Borowski, Kimberly Stencel.
Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts / Meng-Jiao Shi, Maria Borowski, Kimberly Stencel.
Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski.
Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski.
Harvesting Human Embryonic Stem Cells for Cryopreservation / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski.
Human Embryonic Stem Cell Culture on BD Matrigel₉ with mTeSRʼ1 Medium / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski.
Section III: Laboratory Guide for Human Stem Cell Culture: Characterization of Pluripotent Stem Cells. Defining Pluripotency / Kelly P Smith, Mai X Luong.
Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy / Shirwin M Pockwinse, Prachi N Ghule.
Preparation of Human Embryonic Stem Cell Samples for Flow Cytometry / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski.
Characterization of Human Embryonic Stem Cell Gene Expression Using Reverse Transcription Polymerase Chain Reaction / Alicia Allaire, Kelly P Smith, Mai X Luong.
Determining Pluripotency of Human Embryonic Stem Cells: Embryoid Body Formation / Shirwin M Pockwinse, Prachi N Ghule.
Characterization of Human Embryonic Stem Cells by Cytogenetics: Karyotyping and Fluorescence Hybridization / Shirwin M Pockwinse, Prachi N Ghule, Anne Higgins.
High-Resolution Chromatin Immunoprecipitation Assay / Beatriz P̌rez-Cadah̕a, Bojan Drobic, James R Davie.
Assaying Pluripotency Via Teratoma Formation / M William Lensch, Tan A Ince.
Section IV: Perspectives in Human Stem Cell Technologies. Genomic Analysis of Pluripotent Stem Cells / David Lapointe.
Proteomic Analysis of Human Pluripotent Cells / Andy T Y Lau, Yan-Ming Xu, Jen-Fu Chiu.
Bioinformatics Strategies for Understanding Gene Expression in Human Pluripotent Cells / Gustavo Glusman, Bruz Marzolf, Kai Wang, Ji-Hoon Cho, Burak Kutlu, Qiang Tian.
Epigenetic Analysis of Pluripotent Cells / Mojgan Rastegar, Genevïve P Delcuve, James R Davie.
Differentiation of Pluripotent Stem Cells: An Overview / Jeremy Micah Crook.
Cellular Reprogramming: Current Technology, Perspectives, and Generation of Induced Pluripotent Cells / Tanja Dominko.
Section V: Applications of Human Embryonic Stem Cells. Human Pluripotent Cells: The Biology of Pluripotency / Li-Fang Chu, Thomas P Zwaka.
Human Pluripotent Cells for Regenerative Medicine: Potential Applications for Regenerative Medicine / Christopher C Ford, Darrell N Kotton.
Therapeutic Applications of Human Embryonic Stem Cells / Shi-Jiang Lu, Irina Klimanskaya, Edmund Mickunas, Robert Lanza.
Human Stem Cells for Drug Screening, Toxicity, Specificity, and Off-Target Effects / Arnaud Lacoste, Mark Burcin.
Interfacing Stem Cells with Gene Therapy / Christian Mueller, Terence R Flotte.
Tissue Engineering for Stem Cell Mediated Regenerative Medicine / Janet Zoldan, Thomas P Kraehenbuehl, Abigail K R Lytton-Jean, Robert S Langer, Daniel G Anderson.
Glossary.
Index. "Current advances in human stem cell research utilize the latest tools of cell biology, molecular biology, chemistry, biomedical imaging, genomics, proteomics and bioinformatics"--Provided by publisher. "Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers. Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource. A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology. Perspectives written by the world leaders in the field. Introductory chapters will provide background information. The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field"--Provided by publisher.
Open: eu03.alma.exlibrisgroup.com
"Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers. Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource. A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology. Perspectives written by the world leaders in the field. Introductory chapters will provide background information. The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field"--Provided by publisher.