Table of Contents

Archive Tissues.
Preanalytical Time Interval (PATI) and Fixation.
Formalin-Free Fixatives.
Manual Microdissection.
Tissue Microarray (TMA).
Laser Capture Microdissection (LCM).
DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues.
DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells).
Fast Protocol for DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues.
DNA Extraction from Blood and Forensic Samples.
Restoration and Reconstruction of DNA Length.
RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues.
RNA Extraction from Decalcified and Non-decalcified Formalin-Fixed Paraffin-Embedded Tissues.
RNA Temperature Demodification.
MicroRNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues.
Quantification of Nucleic Acids.
Integrity Assessment of Nucleic Acids.
DNase Treatment of RNA.
Reverse Transcription.
General Protocol for End-Point PCR.
Quantitative End-Point PCR.
Nested-PCR.
General Protocol for Dot-Blot.
PCR-ELISA.
Quantitative Real-Time RT-PCR.
DNA Sequencing from PCR Products.
Pyrosequencing Analysis for Detection of KRAS Mutation.
Microsatellite Instability (MSI) Detection in DNA from FFPE Tissues.
General Protocol for Loss of Heterozygosity Detection.
Qualitative Methylation Status Assessment.
Quantitative Methylation Status Assessment in DNA from FFPE Tissues with Bisulfite Modification and Real-Time Quantitative MSP.
Comparative Genomic Hybridisation (CGH).
Multiplex Ligation-dependent Probe Amplification (MLPA).
Fluorescence In Situ Hybridization (FISH) on Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections.
Introduction on Genome-wide Expression Profiling from Formalin-Fixed Paraffin-Embedded Tissues Using Microarrays.
Introduction.
Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissues.
Rapid Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue Samples.
Protein Extraction Protocol from Tissues Treated with Formalin-Free Fixatives.
Trichloroacetic Acid (TCA) Precipitation of Proteins.
One-Dimensional Sodium-Dodecyl-Sulfate (SDS) Polyacrylamide Gel Electrophoresis.
Visualization of Proteins.
Western Blotting.
Dot Blot.
Membrane Stripping.
Reverse-Phase Protein Microarrays.
Introduction.
Sample Preparation and In Gel Tryptic Digestion for Mass Spectrometry Experiments.
MALDI Imaging Mass Spectrometry on Formalin-Fixed Paraffin-Embedded Tissues.
Introduction.
Internal Quality Control (IQC) Organization.
Standards for Equipment and Reagents.