Table of Contents

B. Albertson and R. Swanson, Assays Techniques Available for the Toxicologist. J. Baron, Longitudinal Bone Growth: Experimental Methods. F.G. Berger and G. Watson, Measurement of Steroid Receptor Binding to DNA. G.T. Campbell, M.A. Steele, and C.A. Blake, Hypophysial Grafts Beneath the Renal Capsule: A Model to Study Endocrine Control Systems. F.R. Boockfor, Prolactin Secretion from Single Cells: Analysis by Reverse Hemolytic Plaque Assays. A. Miyauchi, A. Fujimori, and R. Civitelli, Application of Fluorescence Techniques to Bone Biology. E.J. Diamond, Measurement of Thyroid Hormones and Related Molecules. V. Ganapathy, P.D. Prasad, and F.H. Leibach, Uptake Measurements of ThyroidHormones and Amino Acids in Cultured Cells. C. Lee, Y. Qian, and J.A. Sensibar, Two-Dimensional Gel Electrophoresis of Proteins. J.M. McAndrews and S.J. Ringstrom, Effects of in vivo Exposure to Glucorticoids on Pituitary, Serum, and Messenger Ribonucleic Acid Levels of Gonadotropin Hormones FSH and LH in Male and Female Rats. R. Meeker, Glutamate Receptor Autoradiography and in situ Hybridization. W.R. Millington and A.B. Manning, Chromatographic Methods for Analyzing( -Endorphin and Related Peptides. J.S. Dallas and B.S. Prabhakar, Detection of Autoantibodies to the Thyrotropin Receptor. A.S. Meyer and T.J. Schmidt, Measurement of Intracellular Glucocorticoid and Mineralcorticoid Receptors. S.E. Senogles and M.W. Quasney, Use of Transfection Techniques for Studying the Function of the D2 Dopamine Receptors. M.P. Waalkes, Quantitation of Metallothionein. M.M. Weber and P. Michl, Primary Culture of Bovine and Human Adult Adrenocortical Cells. A.M. Moses and C. Jones, Assays for Vasopressin. Subject Index. Endocrine Methods contains descriptions of contemporary and cutting-edge methodologies in various areas of endocrinology, including receptor theory and immunologic techniques for endocrine research. The book presents step-by-step procedures easily available to study the endocrine system and includes experts in their respective fields as contributors. The book presents step-by-step procedures for many important areas of endocrine target organs. Endocrine Methods serves as a valuable methodological resource for investigators using endocrine methods. Includes comprehensive, yet rapid methodical procedures Offers a wide spectrum of assays including both in vivo and in vitro systems important to the several areas of hormone research Describes several techniques for studying receptors, examining osteoblast activity, and measuring parathyroid hormones Encompasses a host of important research tools that can be utilized by the toxicologist and other biomedical scientists.