Table of Contents

Front Matter.
Basics of Flow Cytometry / H Krishnamurthy, L Scott Cram.
Practical Considerations for Flow Cytometric Sorting of Stem Cells / Geoffrey W Osborne.
Stem Cell Analysis and Sorting Using Side Population Analysis / William Telford.
Flow Cytometry in the Study of Proliferation and Apoptosis / Michael G Ormerod, Ronald M Hamelik.
Flow Cytometric Analysis of Drug Transport and Efflux in Stem Cells / Awtar Krishan, Ronald M Hamelik.
Stem Cell Biology and Application / Swapnil Totey, Rajarshi Pal, Murali Krishna Mamidi, Vijayendran Govindasamy, Satish Totey.
Identification and Isolation of Very SmaU Embryonic-like Stem Cells from Murine and Human Specimens / Ewa K Zuba-Surma, Dong-Myung Shin, Habella Klich, Janina Ratajczak, Magda Kucia, Mariusz Z Ratajczak.
Electronic Volume of Hematopoietic Stem Cells / Siddharth Sharma, Awtar Krishan.
Hematopoietic Stem Cells: Issues in Enumeration / Michael Keeney, D Robert Sutherland.
Embryonic Stem Cells: Development and Characterization / Vijay Bhaskar R Konala, Villoo Morawala-Patell, Aparna Khanna.
Human Embryonic Stem Cells: Long-Term Culture and Cardiovascular Differentiation / Maneesha Inamdar.
Mesenchymal Stromal Cells and Their Clinical Applications / Jyoti Kode, Vivek Tanavde.
Circulating Adult Stem Cells of Hematopoietic Origin for Vascular and Neural Regeneration / Lissy K Krishnan.
Flow Cytometric Characterization of Neural Progenitors Derived from Human Pluripotent Stem Cells / Raj R Rao, Sujoy K Dhara, Shilpa Iyer, David W Machacek.
Limbal Stem Cells and Corneal Regeneration / Geeta K Vemuganti, Murali Mohan Sagar Baila, Shubha Tiwari.
Flow Cytometric Sorting of Spermatogonial Stem Cells / B S Srinag, J M Kalappurakkal, GH Mohan, H Krishnamurthy.
Breast Cancer Stem Cells / Devaveena Dey, Annapoorni Rangarajan.
Tumor Stem Cell Marker Expression in Cells from Body Cavity Fluids / Awtar Krishan, Deepti Sharma, Ronald M Hamelik.
Index. A much-needed primer on the use of laser flow cytometry for stem cell analysisLaser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.
A much-needed primer on the use of laser flow cytometry for stem cell analysisLaser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.
Krishan, Awtar;Krishnamurthy, H;Totey, Satish
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Wiley-Blackwell
2010
9780470631119
9780470543986
RefWorks