Table of Contents

Ligand-gated ion channels.
Affinity and efficacy of orthosteric ligands at ligand-gated ion channels.
Competitive interactions between orthostheric ligands at ligand-gated ion channels.
Analysis of allosteric interactions at ligand-gated ion channels.
G protein-coupled receptors and G protein signaling.
Affinity and efficacy of the agonist-receptor-G-protein interaction.
Estimating affinity and efficacy by reverse-engineering and response-clamp analysis.
Analysis of agonism and inverse agonism in signaling pathways that exhibit constitutive activity.
Analysis of ligand bias in receptor signaling through different G protein pathways.
Competitive interactions between orthosteric ligands in functional assays on G protein-coupled receptors.
Analysis of allosterism in functional assays on G protein-coupled receptors.
Analysis of drug-receptor interactions using radioligand binding assays on G protein-coupled receptors.